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Photobleaching Enables Super-resolution Imaging of the FtsZ Ring in the Cyanobacterium Prochlorococcus

6.2K Views

10:09 min

November 6th, 2018

DOI :

10.3791/58603-v

November 6th, 2018


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Photobleaching

Chapters in this video

0:04

Title

0:43

Sample Preparation and Fixation

1:44

Precoating of the Coverslip with Polystyrene Beads and Coating of Poly-L-lysine onto the Bead-coated Coverslip

3:08

Immobilization of Cells on the Coverslip and Permeabilization of Cyanobacterial Cells

4:23

Photobleaching of the Chlorophyll Pigments in a Blocking Step

4:51

Antibody Binding

6:07

Image Acquisition of STORM Data

7:51

Results: Super-resolution Imaging of the FtsZ Ringin in the Cyanobacterium Prochlorococcus

9:42

Conclusion

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