Generating Recombinant Avian Herpesvirus Vectors with CRISPR/Cas9 Gene Editing
13.1K Views
•
12:21 min
•
January 7th, 2019
DOI :
January 7th, 2019
•Chapters in this video
0:04
Title
0:53
CRISPR/Cas9-mediated Knock-in: Transfection and Infection
2:14
Harvesting and Purification of the HVT Recombinant Virus
5:38
Excision of the Fluorescent Reporter Gene via the Cre-lox System
6:23
Plaque Purification
7:31
Verification of the Recombinant HVT
9:31
Stability of the Recombinant Virus
10:36
Results: Analysis of the Generated Recombinant Avian Herpesvirus Vectors
11:38
Conclusion
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