Caffeine Extraction, Enzymatic Activity and Gene Expression of Caffeine Synthase from Plant Cell Suspensions

0:28

Caffeine Extraction in Cell Suspension of C. arabica L.

0:55

Conditions for the Assessment of Caffeine by Thin Layer Chromatography (TLC) Denistometry

1:29

Ribonucleic Acid (RNA) Isolation

2:55

Incubation of RNA Transcript with Deoxyribonuclease (DNase)

3:59

Complementary Deoxyribonucleic Acid (cDNA) Synthesis

5:03

Real-Time Quantitative PCR (qPCR) to Amplify the Gene CCS1

5:43

Total Protein of Cell Suspensions of C. arabica L.

6:26

Enzymatic Assay for Caffeine Synthase

7:39

Results: Enzymatic Activity of Caffeine Synthase Gene Evaluated via qPCR

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Conclusion