Single Droplet Digital Polymerase Chain Reaction for Comprehensive and Simultaneous Detection of Mutations in Hotspot Regions
13.0K Views
•
08:23 min
•
September 25th, 2018
DOI :
September 25th, 2018
•Charles Decraene1,2, Amanda Bortolini Silveira1, Marc Michel1, François-Clément Bidard1,3,4, Jean-Yves Pierga1,3,5, Marc-Henri Stern1,6, Charlotte Proudhon1
1Circulating Tumor Biomarkers Laboratory, SiRIC, Translational Research Department, Institut Curie, PSL Research University, 2CNRS UMR144, Institut Curie, PSL Research University, 3Department of Medical Oncology, Institut Curie, PSL Research University, 4University Versailles Saint-Quentin-en-Yvelines, 5University Paris Descartes, 6Inserm U830, Institut Curie, PSL Research University
Chapters in this video
0:04
Title
0:48
Blood Collection and Plasma Storage
2:04
cfDNA Extraction
4:45
ddPCR Mix Preparation
5:46
Droplet Generation
6:25
PCR Amplification
7:06
Results: KRAS and EGFR Drop-off ddPCR Assays
7:50
Conclusion
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