Contrast-Matching Detergent in Small-Angle Neutron Scattering Experiments for Membrane Protein Structural Analysis and Ab Initio Modeling

0:54

Determine Neutron Contrast Match Points and Necessary Contrast for Protein Measurement

2:05

Express and Purify the Membrane Protein of Interest

3:25

Collect SANS Data and Reduce SANS Data from 2D Image to 1D Plot

4:48

Analyze Data for Structural Parameters of the Scattering Particle

7:06

Create Ab Initio Models from the SANS Data

8:17

Results: Visualization of the Experimental SANS Envelope and Overlay with High-resolution Model using PyMOL

9:40

Conclusion