Flow Cytometry to Estimate Leukemia Stem Cells in Primary Acute Myeloid Leukemia and in Patient-derived-xenografts, at Diagnosis and Follow Up
13.9K Views
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09:01 min
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March 26th, 2018
DOI :
March 26th, 2018
•Thomas Boyer1,2, Fanny Gonzales2,3, Adriana Plesa4, Pauline Peyrouze2, Adeline Barthelemy2, Soizic Guihard2, Bruno Quesnel2,5, Christophe Roumier1,2, Claude Preudhomme1,2, Meyling Cheok2
1Laboratoire d’hématologie, CHU Lille, 2Univ. Lille, Inserm, CHU Lille, UMR-S 1172 - JPArc - Centre de Recherche Jean-Pierre AUBERT Neurosciences et Cancer, 3Service d’hématologie pédiatrique, hôpital Jeanne de Flandre, CHU Lille, 4Laboratoire d’hématologie CH Lyon Sud, 5Service des Maladies du Sang, hôpital Claude Huriez, CHU Lille
Chapters in this video
0:04
Title
0:58
Sample Preparation and Lysis of Red Blood Cells
2:26
Patient Derived Xenografts and Staining
4:50
Flow Cytometry and Gating Strategy
6:14
Results: Parametric Flow Cytometry to Estimate Leukemia Stem Cells in Primary Acute Myeloid Leukemia
7:57
Conclusion
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