Comprehensive Protocol to Sample and Process Bone Marrow for Measuring Measurable Residual Disease and Leukemic Stem Cells in Acute Myeloid Leukemia
29.0K Views
•
09:57 min
•
March 5th, 2018
DOI :
March 5th, 2018
•Jacqueline Cloos*1,2, Jeffrey R. Harris*3, Jeroen J.W.M. Janssen1, Angele Kelder1, F. Huang3, Gerrit Sijm1, Maike Vonk1, Alexander N. Snel1, Jennifer R. Scheick1, Willemijn J. Scholten1, Jannemieke Carbaat-Ham1, Dennis Veldhuizen1, Diana Hanekamp1, Yvonne J.M. Oussoren-Brockhoff1, Gertjan J.L. Kaspers2,4, Gerrit J. Schuurhuis1, A. Kate Sasser3, Gert Ossenkoppele1
Chapters in this video
0:05
Title
1:06
Bone Marrow Aspiration and Smear Preparation
3:16
Flow Cytometry Setup
5:53
Cell Staining for Flow Cytometry
7:45
Results: Representative Leukemia-associated Immunophenotype (LAIP) Gating
8:46
Conclusion
Related Videos
08:00
A Detailed Protocol for Characterizing the Murine C1498 Cell Line and its Associated Leukemia Mouse Model
19.8K Views
08:46
A Protocol for Rapid Post-mortem Cell Culture of Diffuse Intrinsic Pontine Glioma (DIPG)
16.7K Views
09:09
Preparation of Primary Acute Lymphoblastic Leukemia Cells in Different Cell Cycle Phases by Centrifugal Elutriation
8.2K Views
06:14
Moving Upwards: A Simple and Flexible In Vitro Three-dimensional Invasion Assay Protocol
6.0K Views
09:01
Flow Cytometry to Estimate Leukemia Stem Cells in Primary Acute Myeloid Leukemia and in Patient-derived-xenografts, at Diagnosis and Follow Up
13.8K Views
09:02
Immunoglobulin Gene Sequence Analysis In Chronic Lymphocytic Leukemia: From Patient Material To Sequence Interpretation
20.9K Views
06:21
Assessment of the Metabolic Profile of Primary Leukemia Cells
10.2K Views
08:24
Measuring Bone Remodeling and Recreating the Tumor-Bone Microenvironment Using Calvaria Co-culture and Histomorphometry
6.7K Views
09:44
Flow Cytometric Analysis of Mitochondrial Reactive Oxygen Species in Murine Hematopoietic Stem and Progenitor Cells and MLL-AF9 Driven Leukemia
7.2K Views
06:53
Modeling Primary Bone Tumors and Bone Metastasis with Solid Tumor Graft Implantation into Bone
2.6K Views
Copyright © 2025 MyJoVE Corporation. All rights reserved