Measuring Deformability and Red Cell Heterogeneity in Blood by Ektacytometry
14.5K Views
•
09:12 min
•
January 12th, 2018
DOI :
January 12th, 2018
•Nermi L. Parrow*1, Pierre-Christian Violet*2, Hongbin Tu2, James Nichols3, Corinne A. Pittman4, Courtney Fitzhugh4, Robert E. Fleming1,5, Narla Mohandas6, John F. Tisdale3, Mark Levine2
1Department of Pediatrics, Saint Louis University School of Medicine, 2Molecular and Clinical Nutrition Section, Digestive Diseases Branch, National Institute of Diabetes and Digestive and Kidney Diseases, 3Molecular and Clinical Hematology Branch, National Institute of Diabetes and Digestive and Kidney Diseases, 4Sickle Cell Branch, National Heart, Lung and Blood Institute, National Institutes of Health, 5Edward A. Doisy Department of Biochemistry and Molecular Biology, Saint Louis University School of Medicine, 6Red Cell Physiology Laboratory, New York Blood Center
Chapters in this video
0:05
Title
1:05
Ektacytometer Set-up
1:48
Measuring Deformability as a Function of Increasing Shear Stress
4:10
Measuring Cellular Heterogeneity
5:20
Osmotic Gradient Ektacytometry
7:16
Results: Diffraction Pattern Distortion and Relationship to Hemoglobin
8:41
Conclusion
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