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CRISPR/Cas9-mediated Targeted Integration In Vivo Using a Homology-mediated End Joining-based Strategy

14.8K Views

08:22 min

March 12th, 2018

DOI :

10.3791/56844-v

March 12th, 2018


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Keywords Extracted From The Text CRISPR Cas9

Chapters in this video

0:04

Title

0:54

sgRNA Surveyor Nuclease Assay

2:50

Cas9 mRNA Production

4:11

sgRNA Production

5:30

Embryo Collection, Microinjection, and In Vitro Culture

6:43

Results: Superior Integration with Cas9

7:46

Conclusion

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