CRISPR/Cas9-mediated Targeted Integration In Vivo Using a Homology-mediated End Joining-based Strategy
14.8K Views
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08:22 min
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March 12th, 2018
DOI :
March 12th, 2018
•1Institute of Neuroscience, State Key Laboratory of Neuroscience, Key Laboratory of Primate Neurobiology, CAS Center for Excellence in Brain Science and Intelligence Technology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 2College of Life Sciences, University of Chinese Academy of Sciences, 3School of Life Science and Technology, Shanghai Tech University, 4Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences
Chapters in this video
0:04
Title
0:54
sgRNA Surveyor Nuclease Assay
2:50
Cas9 mRNA Production
4:11
sgRNA Production
5:30
Embryo Collection, Microinjection, and In Vitro Culture
6:43
Results: Superior Integration with Cas9
7:46
Conclusion
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