CRISPR/Cas9-mediated Targeted Integration In Vivo Using a Homology-mediated End Joining-based Strategy
14.7K Views
•
08:22 min
•
March 12th, 2018
DOI :
March 12th, 2018
•1Institute of Neuroscience, State Key Laboratory of Neuroscience, Key Laboratory of Primate Neurobiology, CAS Center for Excellence in Brain Science and Intelligence Technology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 2College of Life Sciences, University of Chinese Academy of Sciences, 3School of Life Science and Technology, Shanghai Tech University, 4Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences
Chapters in this video
0:04
Title
0:54
sgRNA Surveyor Nuclease Assay
2:50
Cas9 mRNA Production
4:11
sgRNA Production
5:30
Embryo Collection, Microinjection, and In Vitro Culture
6:43
Results: Superior Integration with Cas9
7:46
Conclusion
Related Videos
11:35
Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells
12.4K Views
10:07
A Standard Methodology to Examine On-site Mutagenicity As a Function of Point Mutation Repair Catalyzed by CRISPR/Cas9 and SsODN in Human Cells
7.7K Views
11:27
Efficient Production and Identification of CRISPR/Cas9-generated Gene Knockouts in the Model System Danio rerio
21.8K Views
09:51
Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
33.6K Views
08:37
A Rapid and Facile Pipeline for Generating Genomic Point Mutants in C. elegans Using CRISPR/Cas9 Ribonucleoproteins
7.5K Views
14:48
Endogenous Protein Tagging in Human Induced Pluripotent Stem Cells Using CRISPR/Cas9
26.0K Views
07:56
Genome Editing in Mammalian Cell Lines using CRISPR-Cas
21.4K Views
09:04
Generation of Defined Genomic Modifications Using CRISPR-CAS9 in Human Pluripotent Stem Cells
8.1K Views
08:23
CIRCLE-Seq for Interrogation of Off-Target Gene Editing
407 Views
10:07
Construction of Homozygous Mutants of Migratory Locust Using CRISPR/Cas9 Technology
2.0K Views
Copyright © 2025 MyJoVE Corporation. All rights reserved