Constitutive and Inducible Systems for Genetic In Vivo Modification of Mouse Hepatocytes Using Hydrodynamic Tail Vein Injection
14.0K Views
•
09:35 min
•
February 2nd, 2018
DOI :
February 2nd, 2018
•Chapters in this video
0:05
Title
0:52
Recombinatorial Cloning to Generate Ready-for-injection Clones
2:23
Hydrodynamic Tail Vein Injection
4:22
Induction of Transfected CreER with Tamoxifen
5:33
Induction of Tetracycline-dependent Gene or shRNA Expression
6:35
Preparation of Mouse Liver for Analysis by Immunostaining
7:30
Results: Constitutive and Inducible Gene Expression in the Liver Following HTVI
8:47
Conclusion
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