An Efficient and Simple Method to Establish NK and T Cell Lines from Patients with Chronic Active Epstein-Barr Virus Infection

9.1K Views

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09:43 min

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March 30th, 2018

DOI :

10.3791/56515-v

March 30th, 2018

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Chongfeng Xu¹, Junhong Ai², Qingxun Zhang¹, Ting Li³, Xiaorong Wu¹, Zhengde Xie², Ziyuan Duan¹

¹Genetic Resources Center, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, ²MOE Key Laboratory of Major Diseases in Children, National Key Discipline of Pediatrics, National Clinical Research Center for Respiratory Diseases, Beijing Key Laboratory of Pediatric Respiratory Infection Diseases, Beijing Pediatric Research Institute, Beijing Children's Hospital, Capital Medical University, ³State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences

Summary

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Cell Line Establishment

Chapters in this video

0:04

Title

1:35

Isolation of PBMC from CAEBV Patients

2:59

Expansion of Cells and Numbering the Viable Cells

4:08

Preparation for Cell Phenotyping by Flow Cytometry

5:52

Expansion and Cryopreservation of NK/T Cells

7:23

Results: Representative Gating Strategy for Flow Cytometry Analysis of Cell Lines L196 and M296

8:25

Conclusion

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