Target Cell Pre-enrichment and Whole Genome Amplification for Single Cell Downstream Characterization
8.8K Views
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10:12 min
•
May 15th, 2018
DOI :
May 15th, 2018
•Shukun Chen*1, Amin El-Heliebi*1, Julia Schmid1, Karl Kashofer2, Zbigniew T. Czyż3, Bernhard Michael Polzer3, Klaus Pantel4, Thomas Kroneis1,5, Peter Sedlmayr1
1Institute of Cell Biology, Histology and Embryology, Medical University of Graz, 2Institute of Pathology, Medical University of Graz, 3Fraunhofer Institute for Toxicology and Experimental Medicine ITEM, 4Department of Tumor Biology, University Medical Center Hamburg-Eppendorf, 5Sahlgrenska Cancer Center, University of Gothenburg
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Chapters in this video
0:05
Title
1:28
Preparation of Target Cells
2:41
Charging the Wire
3:27
Counting Cells on the Wire
4:03
Detachment and Recovery of Target Cells
5:25
Single-cell Sampling Using Micromanipulation
6:37
Single-cell Sampling Using Laser Microdissection
7:47
Results: Use of Functionalized Wire for Ex Vivo Characterization and Genomic Analysis at Single Cell Level
8:59
Conclusion
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