Detecting Estrogenic Ligands in Personal Care Products using a Yeast Estrogen Screen Optimized for the Undergraduate Teaching Laboratory
13.0K Views
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14:13 min
•
January 1st, 2018
DOI :
January 1st, 2018
•Thea M. Edwards1,2, Howard E. Morgan2,3, Coralia Balasca4, Naveen K. Chalasani5, Lauren Yam4,6, Alison M. Roark4
1Department of Biology, University of the South, 2School of Biological Sciences, Louisiana Tech University, 3School of Medicine, Louisiana State University Health Sciences Center, 4Department of Biology, Furman University, 5Department of Computer Science, Louisiana Tech University, 6Clemson University
Chapters in this video
0:05
Title
1:02
Preparing Reagents, Samples, and Extraction Controls
3:23
Subculturing Yeast and Preparing YES Plates
7:18
Processing YES Plates (Day 2 of the Assay)
9:26
Calculating LacZ Values
10:28
Interpolating Sample Estradiol Equivalents (EEQs) using 4-parameter Logistic Regression
11:30
Results: Yeast Estrogen Screen Quantifies Ligands in Personal Care Products (PCPs)
12:52
Conclusion
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