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Protocol for the Differentiation of Human Induced Pluripotent Stem Cells into Mixed Cultures of Neurons and Glia for Neurotoxicity Testing

23.0K Views

09:02 min

June 9th, 2017

DOI :

10.3791/55702-v

June 9th, 2017


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Human Induced Pluripotent Stem Cells

Chapters in this video

0:05

Title

1:10

Human Induced Pluripotent Stem Cell (hiPSC) Expansion

2:20

HiPSC Differentiation into Mixed Neurons and Glia: Generation of Embryoid Bodies

3:41

Generation of Neuropithelial Aggragates

4:53

Aggregate Dissociation and Neuronal Differentiation

6:36

Results: Differentiation of IMR90-hiPSCs into Mixed Cultures of Neurons and Glia

7:53

Conclusion

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