Expression of Exogenous Cytokine in Patient-derived Xenografts via Injection with a Cytokine-transduced Stromal Cell Line
9.3K Views
•
12:58 min
•
May 10th, 2017
DOI :
May 10th, 2017
•Chapters in this video
0:05
Title
1:22
Culture and Expansion of Cytokine Transduced Stromal Cells
3:05
Phospho-flow Cytometry Analysis of Stroma-produced Cytokine Activity
5:09
Injection of Cytokine-transduced Stroma into Mice
6:22
Serial Blood Collection and Plasma Monitoring of Exogenous Human Cytokine in Mice
8:33
Human Hematopoietic Cell Transplantation
9:53
Results: Achieving Physiological Levels of the Human TSLP Cytokine in PDX Mice
11:22
Conclusion
Related Videos
07:20
Ex vivo Expansion of Tumor-reactive T Cells by Means of Bryostatin 1/Ionomycin and the Common Gamma Chain Cytokines Formulation
14.7K Views
06:03
Orthotopic Transplantation of Syngeneic Lung Adenocarcinoma Cells to Study PD-L1 Expression
16.7K Views
10:29
Induction of Graft-versus-host Disease and In Vivo T Cell Monitoring Using an MHC-matched Murine Model
15.6K Views
08:17
A Human Peripheral Blood Mononuclear Cell (PBMC) Engrafted Humanized Xenograft Model for Translational Immuno-oncology (I-O) Research
14.2K Views
06:16
Generation of Orthotopic Pancreatic Tumors and Ex vivo Characterization of Tumor-Infiltrating T Cell Cytotoxicity
12.5K Views
06:06
Induction and Scoring of Graft-Versus-Host Disease in a Xenogeneic Murine Model and Quantification of Human T Cells in Mouse Tissues using Digital PCR
7.0K Views
08:05
Bone Marrow Transplantation Platform to Investigate the Role of Dendritic Cells in Graft-versus-Host Disease
7.7K Views
08:23
Xenograft Skin Model to Manipulate Human Immune Responses In Vivo
2.8K Views
05:00
Ex Vivo T Cell Stimulation in the Pancreatic Tumor: A Method to Increase Cytokine Production in T Cells
1.2K Views
01:37
Evaluation of Stromal Cytokine-Induced Intracellular Protein Phosphorylation Using Phospho-Flow Cytometry
137 Views
Copyright © 2025 MyJoVE Corporation. All rights reserved