Intracellular Staining and Flow Cytometry to Identify Lymphocyte Subsets within Murine Aorta, Kidney and Lymph Nodes in a Model of Hypertension

18.1K Views

•

09:20 min

•

January 28th, 2017

DOI :

10.3791/55266-v

January 28th, 2017

•

Fanny Laroumanie¹, Bethany L. Dale², Mohamed A. Saleh³, Meena S. Madhur¹

¹Department of Medicine, Division of Clinical Pharmacology, Vanderbilt University Medical Center, ²Department of Molecular Physiology and Biophysics, Vanderbilt University, ³Department of Pharmacology and Toxicology, Faculty of Pharmacy, Mansoura University

Summary

Explore More Videos

Keywords Intracellular Staining

Single cell Suspension

Immune Cells

Cardiovascular Disease

Perfusion

Dissection

Tissue Dissociation

Density Gradient Centrifugation

Chapters in this video

0:05

Title

0:46

Kidney, Aorta, and Lymph Node Isolation and Single Cell Suspension Generation

4:29

Ex Vivo Stimulation and Intracellular Cytokine Detection

7:07

Results: Representative Analyses of Kidney, Aorta, and Aortic Lymph Node T Cell Activation

8:23

Conclusion

Related Videos

08:01

Non-surgical Intratracheal Instillation of Mice with Analysis of Lungs and Lung Draining Lymph Nodes by Flow Cytometry

57.3K Views

15:15

Flow Cytometry Analysis of Immune Cells Within Murine Aortas

34.4K Views

14:01

Human In Vitro Suppression as Screening Tool for the Recognition of an Early State of Immune Imbalance

14.5K Views

14:29

Examination of Thymic Positive and Negative Selection by Flow Cytometry

21.7K Views

05:47

Isolation of Murine Lymph Node Stromal Cells

31.0K Views

06:13

Image-based Flow Cytometry Technique to Evaluate Changes in Granulocyte Function In Vitro

12.4K Views

07:14

Phenotypic Characterization of Macrophages from Rat Kidney by Flow Cytometry

14.6K Views

07:14

A CFSE-based Assay to Study the Migration of Murine Skin Dendritic Cells into Draining Lymph Nodes During Infection with Mycobacterium bovis Bacille Calmette-Guérin

12.0K Views

09:45

Detection of Trypanosoma brucei Variant Surface Glycoprotein Switching by Magnetic Activated Cell Sorting and Flow Cytometry

8.7K Views

08:22

Utilization of Capsules for Negative Staining of Viral Samples within Biocontainment

10.1K Views