The Use of Induced Somatic Sector Analysis (ISSA) for Studying Genes and Promoters Involved in Wood Formation and Secondary Stem Development
8.7K Views
•
09:54 min
•
October 5th, 2016
DOI :
October 5th, 2016
•Antanas Spokevicius1, Lynette Taylor1, Emma Melder1, Kim Van Beveren1, Josquin Tibbits2, Nicky Creux3,4, Gerd Bossinger1
1School of Ecosystem and Forest Sciences, Faculty of Science, The University of Melbourne, 2Victorian AgriBiosciences Centre, La Trobe University R&D Park, 3College of Biological Sciences, Department of Plant Biology, University of California, Davis, 4Department of Genetics, Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria
Chapters in this video
0:05
Title
0:46
Preparation of Agrobacterium tumefaciens for Inoculation
1:59
Inoculation of Plant Stems with A. tumefaciens
3:44
Harvesting for GUS Histological Assay
5:57
Identification of Transgenic Tissue
7:22
Results: Successful Transformation of Stem Tissue Using the ISSA Method
9:12
Conclusion
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