Induction of Murine Intestinal Inflammation by Adoptive Transfer of Effector CD4+CD45RBhigh T Cells into Immunodeficient Mice
16.3K Views
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08:37 min
•
April 21st, 2015
DOI :
April 21st, 2015
•1Center for Gastrointestinal Biology and Disease, University of North Carolina at Chapel Hill, 2Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, 3Department of Genetics, University of North Carolina at Chapel Hill, 4Curriculum in Genetics and Molecular Biology, University of North Carolina at Chapel Hill
Chapters in this video
0:05
Title
1:18
Isolation of Splenic T Cells from Donor Mice
2:41
Enrichment of CD4+ T Cells
4:34
Labeling and Sorting the CD4+ T Cells
6:31
Injection of Cells into Recipient Mice and Monitoring of Disease Progression
7:22
Results: Colitis Progression After Adoptive T Cell Transfer into Recipient Mice
8:15
Conclusion
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