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Biochemical and High Throughput Microscopic Assessment of Fat Mass in Caenorhabditis Elegans

20.6K Views

16:07 min

March 30th, 2013

DOI :

10.3791/50180-v

March 30th, 2013


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Caenorhabditis Elegans

Chapters in this video

0:05

Title

2:06

Stamp Frozen Library Plates onto Rectangular Amp/Tet LB Agar Plates

3:16

Grow and Seed RNAi Bacterial Clones and Add Worms to RNAi Plates

5:49

Fix and Stain Worms in Nile Red and Image on a High-throughput Scope

7:22

Wash Worms and Image on a High-throughput Microscope

8:31

Biochemical Lipid Determination Using Solid Phase Extraction and Gas Chromatography-mass Spectrometry

12:02

Representative Fat Mass Content Results

15:39

Conclusion

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