JoVE Logo

Sign In

Identification and Isolation of Slow-Dividing Cells in Human Glioblastoma Using Carboxy Fluorescein Succinimidyl Ester (CFSE)

14.0K Views

11:54 min

April 29th, 2012

DOI :

10.3791/3918-v

April 29th, 2012


Explore More Videos

Slow dividing Cells

Chapters in this video

0:02

Title

0:44

Introduction

1:43

Preparing Glioblastoma Single Cell Suspension

3:06

Staining with Cell Trace Carboxyfluorescein Succinimidyl Ester (CFSE) Green Fluorescent Dye

5:37

Flow Cytometry Analysis of CFSE Stained Glioblastoma Cells

6:44

Isolation of the Slow-dividing Cell Population using Fluorescent Activated Cell Sorting (FACS)

10:02

Representative Results

11:12

Conclusion

Related Videos

article

10:28

Flow Cytometry-based Drug Screening System for the Identification of Small Molecules That Promote Cellular Differentiation of Glioblastoma Stem Cells

8.2K Views

article

07:52

Fluorescence Molecular Tomography for In Vivo Imaging of Glioblastoma Xenografts

8.8K Views

article

09:36

Live-Cell Imaging Assays to Study Glioblastoma Brain Tumor Stem Cell Migration and Invasion

10.1K Views

article

09:24

Isolation and Expansion of Human Glioblastoma Multiforme Tumor Cells Using the Neurosphere Assay

29.7K Views

article

10:08

Co-culture of Glioblastoma Stem-like Cells on Patterned Neurons to Study Migration and Cellular Interactions

5.7K Views

article

12:52

Isolation and Flow Cytometric Analysis of Glioma-infiltrating Peripheral Blood Mononuclear Cells

15.7K Views

article

06:41

Identification of Quiescent Cells in a Zebrafish T-Cell Acute Lymphoblastic Leukemia Model Using Cell Proliferation Staining

658 Views

article

08:08

Isolation and Activation of Murine Lymphocytes

21.0K Views

article

08:35

A Human Glioblastoma Organotypic Slice Culture Model for Study of Tumor Cell Migration and Patient-specific Effects of Anti-Invasive Drugs

12.7K Views

article

09:29

Live Imaging of Microtubule Dynamics in Glioblastoma Cells Invading the Zebrafish Brain

2.6K Views

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2025 MyJoVE Corporation. All rights reserved