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Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples

15.4K Views

13:21 min

May 4th, 2012

DOI :

10.3791/3791-v

May 4th, 2012


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Chemically blocked Antibody Microarray

Chapters in this video

0:05

Title

2:19

Printing the Antibody Microarray

3:58

Chemically Blocking the Antibody Microarray

6:06

Blocking Non-specific Binding with BSA

6:47

Wax Imprinting

7:11

Applying Serum Samples

8:19

Applying Biotinylated Glycan-binding Protein

9:00

Applying Dye-labeled NeutrAvidin and Fluorescence Detection

10:03

Data Extraction and Analysis

12:08

Conclusion

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