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Human In Vitro Suppression as Screening Tool for the Recognition of an Early State of Immune Imbalance

14.5K Views

14:01 min

July 22nd, 2011

DOI :

10.3791/3071-v

July 22nd, 2011


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Human In Vitro Suppression

Chapters in this video

0:05

Title

2:08

Tosylactivated Bead Coating

4:25

PBMC Isolation

5:41

MACS Pre-sort of CD4 T Cells

8:05

Fluorescent Activated Cell Sorting (FACS) Isolation

9:59

Cell Culture Setup, Harvesting, and Counting

11:32

Tregs Differ in their Ability to Successfully Suppress Naive and in vivo-activated T Cells

13:34

Conclusion

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