Quantitative Assessment of Immune Cells in the Injured Spinal Cord Tissue by Flow Cytometry: a Novel Use for a Cell Purification Method
22.1K Views
•
08:00 min
•
April 9th, 2011
DOI :
April 9th, 2011
•1Institute for Memory Impairments and Neurological Disorders, University of California, 2Physical Medicine & Rehabilitation, University of California, 3Anatomy & Neurobiology, University of California, 4Sue and Bill Gross Stem Cell Research Center, University of California, 5Section of Molecular Biology, University of California, 6Reeve-Irvine Research Center, University of California
Explore More Videos
Chapters in this video
0:05
Title
1:23
Dissociation of Spinal Cord Tissue
2:47
Creating OptiPrep Gradient Solutions and Separating Lipid/Myelin Debris from Cells
4:27
Immunolabeling of Specific Immune Cells and Flow Cytometric Quantitative Assessment
6:16
Removal of Lipid/Myelin Debris Enhances Quantitative Detection of Cellular Inflammation in the Injured Spinal Cord Tissue by Flow Cytometry
7:33
Conclusion
Related Videos
09:58
Isolation of Brain and Spinal Cord Mononuclear Cells Using Percoll Gradients
40.6K Views
12:42
Stereological and Flow Cytometry Characterization of Leukocyte Subpopulations in Models of Transient or Permanent Cerebral Ischemia
15.5K Views
12:14
Isolation and Flow Cytometric Analysis of Immune Cells from the Ischemic Mouse Brain
34.8K Views
08:47
Induction of Experimental Autoimmune Encephalomyelitis in Mice and Evaluation of the Disease-dependent Distribution of Immune Cells in Various Tissues
23.5K Views
13:05
Flow Cytometry Protocols for Surface and Intracellular Antigen Analyses of Neural Cell Types
52.0K Views
09:38
Determining Immune System Suppression versus CNS Protection for Pharmacological Interventions in Autoimmune Demyelination
12.1K Views
12:34
Analysis of Immune Cells in Single Sciatic Nerves and Dorsal Root Ganglion from a Single Mouse Using Flow Cytometry
11.4K Views
08:21
High-Dimensionality Flow Cytometry for Immune Function Analysis of Dissected Implant Tissues
2.1K Views
09:01
Flow Cytometric Analysis of Lymphocyte Infiltration in Central Nervous System during Experimental Autoimmune Encephalomyelitis
7.0K Views
09:35
Isolating Central Nervous System Tissues and Associated Meninges for the Downstream Analysis of Immune cells
9.0K Views
Copyright © 2025 MyJoVE Corporation. All rights reserved