Generation of Induced Pluripotent Stem Cells by Reprogramming Human Fibroblasts with the Stemgent Human TF Lentivirus Set
29.8K Views
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12:10 min
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December 8th, 2009
DOI :
December 8th, 2009
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Chapters in this video
0:00
Title
0:09
Introduction
1:05
Preparing Mouse Embryonic Fibroblast (MEF) Conditioned Medium
2:39
Transducing Human-Foreskin-Fibroblast (BJ) Cells with Stem-Cell-Specific Transcription Factors
6:33
Selecting and Passaging Induced Pluripotent Stem Cell Colonies
7:33
Staining Resulting Colonies for Markers of Pluripotency
9:43
Representative Results: Induced Pluripotent Stem Cell Colonies Morphologically Resemble Stem-Cells and Express Markers of Pluripotency
11:07
Conclusion
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