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The University of Arizona College of Medicine

1 ARTICLES PUBLISHED IN JoVE

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Biochemistry

Live Cell Imaging with Time Lapse Photography to Study Epidermal Keratinocyte Proliferation Kinetics
Brook Abegaze 1, Giselle Vitcov 2, Richard Kim 3, Ruby Ghadially 1
1The University of California, San Francisco, and San Francisco Veterans Affairs Health Care System, 2The University of Arizona College of Medicine, 3Department of Medicine, Memorial Sloan Kettering Cancer Center

Here, we provide a method for live cell imaging analysis that can be used to manually track the lineages of passage 0 keratinocytes and that allows the collection of proliferation metrics, including cell division fate and cell cycle duration.

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