A Unique Mouse Model for Quantitative Assessment of Biofilm Formation on Surgical Implants in Subcutaneous AbscessMitsuhiro Nishizawa 1, Bahram Saleh 2, Ralph Marcucio *1, Kazuhito Morioka *1,3
1Department of Orthopaedic Surgery, Orthopaedic Trauma Institute (OTI), University of California, San Francisco (UCSF), 2Rosies Base, LLC, 3Department of Neurological Surgery, Weill Institute for Neurosciences, Brain and Spinal Injury Center (BASIC), University of California, San Francisco (UCSF)
This protocol describes a unique experimental model of implant-related infections that enables the simultaneous incubation of two implants with bacteria under identical conditions within a single mouse. It also allows precise assessment of biofilm formation on implant surfaces using optimized comparative analytical methods, demonstrating advanced techniques for evaluating biomaterials' antimicrobial properties.
