Laboratorio de Bioquímica y Biotecnología de Hongos, Carpio y plan de Ayala s/n, Col. Santo Tomás

1 ARTICLES PUBLISHED IN JoVE

Bioengineering

Design of Solid-State Fermentation Systems for Polymer Hydrolytic Extracellular Enzyme Production by Filamentous Fungi

Cynthia Lizbeth López-García¹, Adriana Jazmín Legorreta-Castañeda¹, Raúl Sansón-Temich¹, Guadalupe Guerra-Sánchez¹, Dario Rafael Olicón-Hernández¹

¹Instituto Politécnico Nacional, Escuela Nacional de Ciencias Biológicas, Departamento de Microbiología, Laboratorio de Bioquímica y Biotecnología de Hongos, Carpio y plan de Ayala s/n, Col. Santo Tomás

This protocol utilizes wheat bran in a rotary solid-state fermentation system to enhance enzyme production. The substrate, supplemented with inducers such as chitin, supports fungal growth under controlled conditions. Results demonstrate enzyme yields 4-6 times higher compared to submerged fermentation, showcasing the method's adaptability and effectiveness for diverse biotechnological applications.