We present a protocol for the isolation, culture, and adipogenic induction of neural crest derived adipose-derived stem cells (NCADSCs) from the periaortic adipose tissue of Wnt-1 Cre+/-;Rosa26RFP/+ mice. The NCADSCs can be an easily accessible source of ADSCs for modeling adipogenesis or lipogenesis in vitro.
This study presents a successful technique using a titanium clip and dental floss traction-assisted endoscopic submucosal dissection for the treatment of early gastric cancer.
Here, we present a protocol for using polysaccharide hemostatic materials to manage hemorrhage after endoscopic submucosal dissection (ESD).
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