Generation and Characterization of Human Induced Pluripotent Stem Cell-derived Astrocytes Lacking Fragile X Messenger RibonucleoproteinBharath Kumar Reddy 1,4, Nikhita Annaiyappa 2, Aditi Bhattacharya 2, Sumantra Chattarji 1, Rakhi Pal 1,3,4
1Centre for High Impact Neuroscience and Translational Applications, TCG Centres for Research and Education in Science and Technology, 2Centre for Brain Development and Repair, 3National Centre for Biological Sciences, Tata Institute for Fundamental Research, 4Academy of Scientific and Innovative Research (AcSIR)
Here we describe a protocol that facilitates the differentiation of human induced pluripotent stem cells into functional forebrain-specific astrocytes. This enables investigations into the role of glial cells in the pathogenesis of neurodevelopmental disorders, such as Fragile X Syndrome, and modeling of other brain disorders.
