Tata Institute for Fundamental Research

1 ARTICLES PUBLISHED IN JoVE

Neuroscience

Generation and Characterization of Human Induced Pluripotent Stem Cell-derived Astrocytes Lacking Fragile X Messenger Ribonucleoprotein

Bharath Kumar Reddy^1,4, Nikhita Annaiyappa², Aditi Bhattacharya², Sumantra Chattarji¹, Rakhi Pal^1,3,4

¹Centre for High Impact Neuroscience and Translational Applications, TCG Centres for Research and Education in Science and Technology, ²Centre for Brain Development and Repair, ³National Centre for Biological Sciences, Tata Institute for Fundamental Research, ⁴Academy of Scientific and Innovative Research (AcSIR)

Here we describe a protocol that facilitates the differentiation of human induced pluripotent stem cells into functional forebrain-specific astrocytes. This enables investigations into the role of glial cells in the pathogenesis of neurodevelopmental disorders, such as Fragile X Syndrome, and modeling of other brain disorders.