Efficient PAM-Less Base Editing for Zebrafish Modeling of Human Genetic Disease with zSpRY-ABE8e
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07:31 min
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February 17th, 2023
DOI :
February 17th, 2023
•1Key Laboratory of Brain, Cognition and Education Science, Ministry of Education, 2Institute for Brain Research and Rehabilitation, Guangdong Key Laboratory of Mental Health and Cognitive Science, South China Normal University, 3Institute of Modern Aquaculture Science and Engineering, Guangdong Provincial Engineering Technology Research Center for Environmentally Friendly Aquaculture, Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture, School of Life Sciences, South China Normal University, 4Department of Pathology, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences
Rozdziały w tym wideo
0:04
Introduction
1:02
Preparation of Microinjection Capillaries and Modified RNA
2:23
RNA Microinjection of Zebrafish Embryos
4:36
Efficiency Analysis of EditR Base Edits
6:08
Results: TSR2 Diamond Blackfan Zebrafish Mutation
6:41
Conclusion
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