Isolation of Next-Generation Gene Therapy Vectors through Engineering, Barcoding, and Screening of Adeno-Associated Virus (AAV) Capsid Variants
Please note that all translations are automatically generated. Click here for the English version.
4.4K Views
•
09:20 min
•
October 18th, 2022
DOI :
October 18th, 2022
•Kleopatra Rapti1,2, Olena Maiakovska1,2, Jonas Becker1,2, Joanna Szumska1,2, Margarita Zayas1,2, Felix Bubeck1,2, Jixin Liu1,2, Emma Gerstmann1,2, Chiara Krämer1,2, Ellen Wiedtke1,2, Dirk Grimm1,2,3,4,5
1Department of Infectious Diseases/Virology, Section Viral Vector Technologies, Medical Faculty, University of Heidelberg, 2BioQuant Center, BQ0030, University of Heidelberg, 3Cluster of Excellence CellNetworks, 4German Center for Infection Research (DZIF), 5German Center for Cardiovascular Research (DZHK)
Rozdziały w tym wideo
0:05
Introduction
1:16
Analysis of the AAV Viral Library (In Vitro) by NGS
3:42
Analysis of AAV Viral Library (In Vivo) by NGS
7:08
Results: Peptide Display AAV Capsid Engineering and Barcoded AAV Library Screening for Isolation of Next-Generation Gene Therapy Vectors
8:36
Conclusion
Powiązane Filmy
21:55
Engineering and Evolution of Synthetic Adeno-Associated Virus (AAV) Gene Therapy Vectors via DNA Family Shuffling
28.4K Views
10:03
Transplantation of Induced Pluripotent Stem Cell-derived Mesoangioblast-like Myogenic Progenitors in Mouse Models of Muscle Regeneration
9.5K Views
13:31
Novel Atomic Force Microscopy Based Biopanning for Isolation of Morphology Specific Reagents against TDP-43 Variants in Amyotrophic Lateral Sclerosis
8.7K Views
07:09
Isolation of Murine Adipose Tissue-derived Microvascular Fragments as Vascularization Units for Tissue Engineering
12.4K Views
09:21
Production, Purification, and Quality Control for Adeno-associated Virus-based Vectors
35.8K Views
07:21
Production of Adeno-Associated Virus Vectors in Cell Stacks for Preclinical Studies in Large Animal Models
5.9K Views
09:12
Isolation of Adeno-Associated Viral Vectors Through a Single-Step and Semi-Automated Heparin Affinity Chromatography Protocol
2.7K Views
05:51
A Streamlined and Standardized Procedure for Generating High-Titer, High-Quality Adeno-Associated Virus Vectors Utilizing a Cell Factory Platform
1.4K Views
04:43
Quantification of Adeno-Associated Viral Genomes in Purified Vector Samples by Digital Droplet Polymerase Chain Reaction
1.7K Views
03:52
Suspension Culture Production and Purification of Adeno-Associated Virus by Iodixanol Density Gradient Centrifugation for In Vivo Applications
2.1K Views
Copyright © 2025 MyJoVE Corporation. Wszelkie prawa zastrzeżone