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Efficient Transfection of In vitro Transcribed mRNA in Cultured Cells Using Peptide-Poloxamine Nanoparticles

3.1K Views

10:16 min

August 17th, 2022

DOI :

10.3791/64288-v

August 17th, 2022


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Transfection

Rozdziały w tym wideo

0:05

Introduction

1:19

In Vitro Transcription (IVT) of Chemically Modified mRNA

2:55

Generation of IVT mRNA/PP-sNps

5:22

Preparation of the Cells for Transfection and Transfection of the Cultured Cells

7:04

Analysis of Cell Transfection Efficacy Using Metridia Luciferase (MetLuc) Assay

7:53

Results: Analyzing the Transfection Efficiency of MetLuc-mRNA/PP-sNp

9:12

Conclusion

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