The Peel-Blot Technique: A Cryo-EM Sample Preparation Method to Separate Single Layers From Multi-Layered or Concentrated Biological Samples
Please note that all translations are automatically generated. Click here for the English version.
2.2K Views
•
07:27 min
•
June 29th, 2022
DOI :
June 29th, 2022
•Rozdziały w tym wideo
0:04
Introduction
0:33
Preparation Steps for the Peel‐Blot Technique
1:57
Placing the Carbon Film onto the Grid
3:07
Separation of Multi-Layered 2D Crystals into Single Layer
6:42
Conclusion
4:55
Results: 2D Crystals of Human Leukotriene C4 Synthase and Liposomes Subjected to the Peel‐Blot Method, the Peel-Blot Footprint, and Blotting an EM Grid Prepared by Back-Injection on a Submicron Filter Paper
Powiązane Filmy
09:33
High-Resolution Complexome Profiling by Cryoslicing BN-MS Analysis
7.1K Views
06:12
Mechanical Separation and Protein Solubilization of the Outer and Inner Perivitelline Sublayers from Hen's Eggs
4.3K Views
09:00
A Sample Preparation Pipeline for Microcrystals at the VMXm Beamline
2.8K Views
11:52
Single Particle Cryo-Electron Microscopy: From Sample to Structure
8.2K Views
09:16
Manual Blot-and-Plunge Freezing of Biological Specimens for Single-Particle Cryogenic Electron Microscopy
6.1K Views
08:17
Sample Preparation for In Situ Cryotomography of Mammalian Cells
2.8K Views
12:52
Tandem High-pressure Freezing and Quick Freeze Substitution of Plant Tissues for Transmission Electron Microscopy
16.3K Views
11:08
Two Peeling Methods for the Isolation of Photoreceptor Cell Compartments in the Mouse Retina for Protein Analysis
3.0K Views
06:24
Cryo-section Dissection of the Adult Subependymal Zone for Accurate and Deep Quantitative Proteome Analysis
3.3K Views
09:04
Miniaturized Sample Preparation for Transmission Electron Microscopy
19.5K Views
Copyright © 2025 MyJoVE Corporation. Wszelkie prawa zastrzeżone