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Residue-Specific Exchange of Proline by Proline Analogs in Fluorescent Proteins: How "Molecular Surgery" of the Backbone Affects Folding and Stability

2.8K Views

10:31 min

February 3rd, 2022

DOI :

10.3791/63320-v

February 3rd, 2022


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Proline Analogs

Rozdziały w tym wideo

0:04

Introduction

1:39

Production of Recombinant Wild-Type Fluorescent Proteins and Selective Pressure Incorporation to Produce Fluorescent Proteins with Proline Analogs

4:08

Fluorescence Emission of Protein Variants

5:00

Denaturation and Refolding of EGFP Variants

6:38

Results: Effect of Atomic Substitutions in Variants of GFP

9:17

Conclusion

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