A Microphysiological System to Study Leukocyte-Endothelial Cell Interaction during Inflammation
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3.2K Views
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12:55 min
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December 9th, 2021
DOI :
December 9th, 2021
•Qingliang Yang1, Jordan C. Langston2, Yuan Tang3, Balabhaskar Prabhakarpandian4, Laurie E. Kilpatrick5, Mohammad F. Kiani1,2,6
1Department of Mechanical Engineering, Temple University, 2Department of Bioengineering, Temple University, 3Department of Bioengineering, University of Toledo, 4Biomedical Technology, CFD Research Corporation, 5Center for Inflammation and Lung Research, Department of Microbiology, Immunology and Inflammation, Lewis Katz School of Medicine, Temple University, 6Department of Radiation Oncology, Lewis Katz School of Medicine, Temple University
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Rozdziały w tym wideo
0:04
Introduction
1:13
Priming and Coating the Device with Human Fibronectin
3:01
Seeding Biomimetic Microfluid Assay (bMFA) with HLMVEC
4:37
Cell Culture Under Flow for 48 Hours
5:26
Cytokine and/or Therapeutic Treatment
6:19
Neutrophil Adhesion and Migration Experiment with bMFA
8:33
Acquire Image
9:59
Results: Biomimetic Microfluid Assay to Study Leukocyte-Endothelial Cell Interactions
12:06
Conclusion
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