Isolation of Murine Retinal Endothelial Cells for Next-Generation Sequencing
Please note that all translations are automatically generated. Click here for the English version.
2.9K Views
•
09:59 min
•
October 11th, 2021
DOI :
October 11th, 2021
•1Department of Cell Biology, University of Virginia School of Medicine, 2Robert M. Berne Cardiovascular Research Center, University of Virginia School of Medicine, 3Department of Cardiology, University of Virginia School of Medicine, 4Hematovascular Biology Center, University of Virginia School of Medicine, 5Yale Cardiovascular Research Center, Yale University School of Medicine
Przeglądaj więcej filmów
Rozdziały w tym wideo
0:04
Introduction
0:38
Obtain Mouse Eyes for Retinal Isolation and Isolate Mouse Retinal Tissue
2:30
Digest Retinal Tissue into a Single Cell Suspension and Immunostain the Cells with Antibodies
4:07
Prepare for Fluorescence‐Activated Cell Sorting
5:19
Isolate Viable Endothelial Cells via FACS
7:18
Results: Analysis of Isolated Endothelial Cells and Next‐Generation Sequencing After Isolation
9:03
Conclusion
Powiązane Filmy
08:56
Isolation of Murine Embryonic Hemogenic Endothelial Cells
11.4K Views
11:01
Isolation of Primary Murine Retinal Ganglion Cells (RGCs) by Flow Cytometry
14.9K Views
07:22
Isolation of Retinal Stem Cells from the Mouse Eye
24.7K Views
11:26
Single-cell RNA-Seq of Defined Subsets of Retinal Ganglion Cells
13.7K Views
09:34
Isolation of Murine Valve Endothelial Cells
14.2K Views
11:08
Two Peeling Methods for the Isolation of Photoreceptor Cell Compartments in the Mouse Retina for Protein Analysis
3.0K Views
05:04
A Simplified Method for Isolation and Culture of Retinal Pigment Epithelial Cells from Adult Mice
958 Views
09:12
Isolation of Endothelial Cells from the Lumen of Mouse Carotid Arteries for Single-Cell Multi-Omics Experiments
3.1K Views
06:21
Isolation of Primary Mouse Retinal Pigmented Epithelium Cells
4.8K Views
05:52
Murine Dermal Lymphatic Endothelial Cell Isolation
1.3K Views
Copyright © 2025 MyJoVE Corporation. Wszelkie prawa zastrzeżone