Liquid Chromatography Coupled to Refractive Index or Mass Spectrometric Detection for Metabolite Profiling in Lysate-based Cell-free Systems

1:08

Starting, Stopping, and Processing Time Course CFME Reactions for HPLC-RID Quantification

3:18

Creating a Sequence Table for Autosampling and Start the HPLC-RID System for Data Acquisition

4:54

Extracting and Analyzing Data Post-Run

7:20

Starting, Stopping, and Processing Time Course Isotope Tracing CFME Reactions for LC-MS/MS Quantification

9:20

Setting Up a Run Sequence and Starting the LC-MS/MS Run

10:16

Calculating Negative Mode Masses of 13C-Labeled Glucose-Derived Metabolites and Searching for the m/z Features of These Analytes in Filtered Data

11:45

Results: Metabolite Profiling in Lysate-based Cell-free Systems

13:54

Conclusion