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Real-Time Fluorescent Measurement of Synaptic Functions in Models of Amyotrophic Lateral Sclerosis

2.5K Views

08:59 min

July 16th, 2021

DOI :

10.3791/62813-v

July 16th, 2021


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Real Time Fluorescent Measurement

Rozdziały w tym wideo

0:04

Introduction

0:52

Microscope and Perfusion System Setup

2:33

Styryl Dye Imaging of Synaptic Vesicle Release

4:30

Fluorescence Imaging of Gcamp6m Calcium Transients

5:36

Image Analysis

6:23

Results: Real-Time Monitoring of the Dynamics of Presynaptic Calcium Influx and Synaptic Vesicle Membrane Fusion in In Vitro Cultured Neurons

8:15

Conclusion

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