Comprehensive Workflow of Mass Spectrometry-based Shotgun Proteomics of Tissue Samples
Please note that all translations are automatically generated. Click here for the English version.
5.1K Views
•
14:51 min
•
November 13th, 2021
DOI :
November 13th, 2021
•Rozdziały w tym wideo
0:00
Introduction
0:42
Tissue Lysate Preparation
1:51
Quantification and Quality Check of Tissue Lysates
2:33
Enzymatic Digestion of Proteins
3:57
Desalting of Digested Peptides
5:16
Quantification of Desalted Peptides
5:58
Labeling of Digested Peptides using iTRAQ Reagents
8:14
Liquid Chromatography Set-up
9:25
Mass Spectrometry Set-up
12:30
Representative Results
13:45
Conclusions
Powiązane Filmy
09:35
Resolving Affinity Purified Protein Complexes by Blue Native PAGE and Protein Correlation Profiling
13.7K Views
10:37
Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
11.8K Views
07:12
A Plasma Sample Preparation for Mass Spectrometry using an Automated Workstation
9.9K Views
07:44
TMT Sample Preparation for Proteomics Facility Submission and Subsequent Data Analysis
12.5K Views
10:12
Shotgun Proteomics Sample Processing Automated by an Open-Source Lab Robot
3.6K Views
08:08
Quantitative Mass Spectrometric Profiling of Cancer-cell Proteomes Derived From Liquid and Solid Tumors
16.0K Views
11:46
Shotgun Lipidomics of Rodent Tissues
1.9K Views
08:59
A Streamlined Approach for Mass Spectrometry-Based Proteomics Using Selected Tissue Regions
197 Views
05:37
Label-Free Quantitative Proteomics Workflow for Discovery-Driven Host-Pathogen Interactions
6.5K Views
High-throughput and Deep-proteome Profiling by 16-plex Tandem Mass Tag Labeling Coupled with Two-dimensional Chromatography and Mass Spectrometry
5.4K Views
Copyright © 2025 MyJoVE Corporation. Wszelkie prawa zastrzeżone