Probing mRNA Kinetics in Space and Time in Escherichia coli using Two-Color Single-Molecule Fluorescence In Situ Hybridization
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7.1K Views
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10:01 min
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July 30th, 2020
DOI :
July 30th, 2020
•1Department of Physics, University of Illinois at Urbana-Champaign, 2Center for the Physics of Living Cells, University of Illinois at Urbana-Champaign, 3Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, 4Center for Biophysics and Quantitative Biology, University of Illinois at Urbana-Champaign
Rozdziały w tym wideo
0:04
Introduction
0:42
Coverslip and Glass Slide Preparation
1:46
Time-Course Experiment and Sample Fixation
3:18
Cell Membrane Permeabilization
3:57
Probe Hybridization
4:31
Imaging
5:25
Post-Hybridization Wash and Imaging Preparation
6:20
Image Analysis
7:36
Results: Representative mRNA Kinetic Probing Analysis
9:22
Conclusion
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