Phosphoproteomic Strategy for Profiling Osmotic Stress Signaling in Arabidopsis
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5.1K Views
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05:47 min
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June 25th, 2020
DOI :
June 25th, 2020
•1Department of Plant Biology, Carnegie Institute for Science, 2Graduate School of Pharmaceutical Sciences, Kyoto University, 3Department of Biochemistry, Purdue University, 4Department of Chemistry, Purdue University, 5Shanghai Center for Plant Stress Biology, CAS Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences
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Rozdziały w tym wideo
0:04
Introduction
0:33
Immobilized Metal Ion Affinity Chromatography (IMAC) and High pH Reversed-Phase (Hp-RP) Stage Tip Preparation
1:10
IMAC Stage Tip Phosphopeptide Enrichment
2:44
Hp-RP C18 Stage Tip Phosphopeptide Fractionation
3:37
Results: Representative Osmotic Stress Signaling in Arabidopsis
5:05
Conclusion
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