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Super-Resolution Imaging to Study Co-Localization of Proteins and Synaptic Markers in Primary Neurons

5.7K Views

14:02 min

October 31st, 2020

DOI :

10.3791/61434-v

October 31st, 2020


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Super resolution Imaging

Rozdziały w tym wideo

0:00

Introduction

1:04

Primary Cultures

1:48

Immunofluorescence Staining

3:32

SIM Microscopy

4:01

Microscope Calibration

7:11

Acquisition

9:24

Post-production: Image Reconstruction

11:24

Colocalization with Profile Analysis

12:05

Quantification of Pearson's and Mander's

12:27

Results

13:16

Conclusions

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