Simultaneous Quantification of Selected Kynurenines Analyzed by Liquid Chromatography-Mass Spectrometry in Medium Collected from Cancer Cell Cultures
3.3K Views
•
10:38 min
•
May 9th, 2020
DOI :
May 9th, 2020
•Rozdziały w tym wideo
0:04
Introduction
0:57
3-Nitrotyrosine (3NT), Kynurenine (Kyn), 3-Hydroxykynurenine (3HKyn), 3-Hydroxyanthranilic Acid (3HAA), Xanthurenic Acid (XA) and Charcoal Treated Culture Medium Preparation
1:58
Calibration Solution Preparation
3:42
Liquid Chromatography-Mass Spectrometry (LC-MS) System Setu
4:20
Calibration Curve Construction
4:55
Sample Collection
5:33
Sample Preparation for LC-MS Analysis
6:30
Protein Content Assessment and Data Normalization
8:12
Results: Representative LC-MS Data of Selected KYNURENINES Determination
9:57
Conclusion
Powiązane Filmy
Cellular Lipid Extraction for Targeted Stable Isotope Dilution Liquid Chromatography-Mass Spectrometry Analysis
15.1K Views
In situ TEM of Biological Assemblies in Liquid
10.0K Views
Study of Cell Migration in Microfabricated Channels
11.8K Views
Quantification of Global Diastolic Function by Kinematic Modeling-based Analysis of Transmitral Flow via the Parametrized Diastolic Filling Formalism
11.1K Views
Sample Preparation Strategies for Mass Spectrometry Imaging of 3D Cell Culture Models
18.1K Views
Generation of Scalable, Metallic High-Aspect Ratio Nanocomposites in a Biological Liquid Medium
9.0K Views
Automated Robotic Liquid Handling Assembly of Modular DNA Devices
12.3K Views
Synthesis of Biocompatible Liquid Crystal Elastomer Foams as Cell Scaffolds for 3D Spatial Cell Cultures
9.4K Views
Production of Genetically Engineered Golden Syrian Hamsters by Pronuclear Injection of the CRISPR/Cas9 Complex
12.8K Views
A Microcontroller Operated Device for the Generation of Liquid Extracts from Conventional Cigarette Smoke and Electronic Cigarette Aerosol
8.3K Views
Copyright © 2025 MyJoVE Corporation. Wszelkie prawa zastrzeżone