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Generation of Defined Genomic Modifications Using CRISPR-CAS9 in Human Pluripotent Stem Cells

8.1K Views

09:04 min

September 25th, 2019

DOI :

10.3791/60085-v

September 25th, 2019


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CRISPR Cas9

Rozdziały w tym wideo

0:04

Title

1:19

Test gRNA (GuidE RNA) Cutting Efficiency

3:50

Clone Screening

4:51

Transfection Setup

6:07

Checking for Mutations in Single Colonies

7:11

Results: Cutting Efficiency, and Generation and Screening of Mutations

8:10

Conclusion

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