JoVE Logo

Zaloguj się

Monitoring GPCR-β-arrestin1/2 Interactions in Real Time Living Systems to Accelerate Drug Discovery

6.8K Views

08:21 min

June 28th, 2019

DOI :

10.3791/59994-v

June 28th, 2019


Przeglądaj więcej filmów

GPCR

Rozdziały w tym wideo

0:04

Title

1:04

Primer Design

1:56

PCR and DNA Digestion

3:23

Isolation of the Finished Plasmid

4:15

Transfection and Protein Expression

5:56

Monitoring Receptor-β-arrestin 1 and 2 Interactions in HEK293 Cells

7:07

Results: GLP-1r-β-arrestin 1 and 2 Orientation Screening and Interactions

7:44

Conclusion

Powiązane Filmy

article

13:45

Real-time Imaging of Leukotriene B4 Mediated Cell Migration and BLT1 Interactions with β-arrestin

12.8K Views

article

14:02

Optimizing the Genetic Incorporation of Chemical Probes into GPCRs for Photo-crosslinking Mapping and Bioorthogonal Chemistry in Live Mammalian Cells

8.4K Views

article

15:13

High-resolution Spatiotemporal Analysis of Receptor Dynamics by Single-molecule Fluorescence Microscopy

11.3K Views

article

09:03

Parallel Interrogation of β-Arrestin2 Recruitment for Ligand Screening on a GPCR-Wide Scale using PRESTO-Tango Assay

12.1K Views

article

12:27

A Pipeline to Investigate the Structures and Signaling Pathways of Sphingosine 1-Phosphate Receptors

3.3K Views

article

08:46

A "Dual-Addition" Calcium Fluorescence Assay for the High-Throughput Screening of Recombinant G Protein-Coupled Receptors

2.1K Views

article

06:03

Real-Time cAMP Dynamics in Live Cells Using the Fluorescent cAMP Difference Detector In Situ

799 Views

article

07:41

A Kinetic Fluorescence-based Ca2+ Mobilization Assay to Identify G Protein-coupled Receptor Agonists, Antagonists, and Allosteric Modulators

8.8K Views

article

07:48

Tracking Drug-induced Changes in Receptor Post-internalization Trafficking by Colocalizational Analysis

8.7K Views

article

09:12

G Protein-selective GPCR Conformations Measured Using FRET Sensors in a Live Cell Suspension Fluorometer Assay

9.6K Views

JoVE Logo

Prywatność

Warunki Korzystania

Zasady

Badania

Edukacja

O JoVE

Copyright © 2025 MyJoVE Corporation. Wszelkie prawa zastrzeżone