Construction of CRISPR Plasmids and Detection of Knockout Efficiency in Mammalian Cells through a Dual Luciferase Reporter System
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6.2K Views
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05:51 min
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December 5th, 2020
DOI :
December 5th, 2020
•Rozdziały w tym wideo
0:05
Introduction
0:45
Oligonucleotide Annealing and sgRNA/CRISPR Vector Digestion
1:51
Identification of the Correct Recombinant Plasmids by PCR
2:42
Dual-luciferase Detection
4:16
Results: Design of sgRNA to Target Sheep DKK2 Exon 1
5:20
Conclusion
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