Proofreading and DNA Repair Assay Using Single Nucleotide Extension and MALDI-TOF Mass Spectrometry Analysis
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9.5K Views
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11:08 min
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June 19th, 2018
DOI :
June 19th, 2018
•Kang-Yi Su*1,2, Steven D. Goodman*3, Hung-Ming Lai1, Rong-Syuan Yen1, Wei-Yao Hu1, Wern-Cherng Cheng2, Liang-In Lin1,2, Ya-Chien Yang1,2, Woei-Horng Fang1,2
1Department of Clinical Laboratory Sciences and Medical Biotechnology, College of Medicine, National Taiwan University, 2Department of Laboratory Medicine, National Taiwan University Hospital, 3Center for Microbial Pathogenesis, Nationwide Children's Hospital and the Department of Pediatrics, The Ohio State University
Rozdziały w tym wideo
0:05
Title
1:17
Proofreading Reactions
3:16
Terminate the Reaction and Transfer Reaction Products to a Matrix Chip
4:54
Setup the Assay Information on the Mass Spectrometry
6:18
MALDI-TOF Mass Spectrometry (MS) Operation and Data Analysis
8:00
Results: DNA Proofreading and Repair Analyzed by MALDI-TOF MS
9:49
Conclusion
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