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Retrospective MicroRNA Sequencing: Complementary DNA Library Preparation Protocol Using Formalin-fixed Paraffin-embedded RNA Specimens

9.3K Views

12:24 min

May 5th, 2018

DOI :

10.3791/57471-v

May 5th, 2018


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MicroRNA

Rozdziały w tym wideo

0:04

Title

0:39

3' Barcoded Adapter Ligations with 18 Individual RNA Samples

2:00

Ligated Small RNA Purification

5:26

5' Ligated and 3' Barcoded Purified Small RNAs Reverse Transcription

8:12

Pilot PCR and Large-scale PCR Amplification

10:46

Results: Protocol Robust Whether Fresh, Frozen, or Aged

12:01

Conclusion

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